SMALP: How to solubilize membrane proteins using synthetic nanodiscs | Cube Biotech

The solubilization & subsequent stabilization of membrane proteins is more straightforward than assumed. Let us show you how few steps are required!

This video shows this through the exemplary use of our SMALP products:
https://cube-biotech.com/products/nan...

Individual steps of the protocol:
0:00 - Intro
0:11 - Mixing SMALP Powder and Protein Lysate
1:41 - Transfer into new 2 mL centrifuge tube
1:51 - Magnetic stirring overnight at 4°C
2:10 - Ultra-centrifugation
3:05 - Take Sample for protein western blot control

The full protocol can be found here (Also as PDF-file):
https://cube-biotech.com/media/36/de/...

If you have more questions regarding this protocol, possible optimization, or our products in general, feel free to write an email to "[email protected]" or use the Chatbot at https://cube-biotech.com/ to get live support.


Cube Biotech's SMALP products use the same polymer backbone as SMALP products by Orbiscope.