Скачать или смотреть Introduction to spheroid | 3D in structure | primary cells & immortalized cell lines

Spheroids, the three-dimensional (3D) cell cultures that arrange themselves during proliferation into sphere-like formations, got their name in the 1970s, when scientists observed that hamster lung cells grown in suspension arranged themselves in a nearly perfect spherical form.

Unlike two-dimensional, monolayer cell cultures, in which cells interact with the substrate they grow on, 3D cell cultures can grow into spheroids that promote cell–to–cell connectivity and can be embedded in the extracellular matrix (ECM) within native tissue. They help provide a better understanding of cells in their microenvironment and offer a more realistic cellular scenario for research.

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