Скачать или смотреть Spatial Transcriptomics of Alzheimer's Disease Mouse Models with and Without Trem2R47H Mutation

Presented By: Frances Edwards, PhD

Speaker Biography: Frances Edwards is a CNS synaptic physiologist. After graduating with a BSc Honours and MSc in Behavioural Pharmacology from University of Sydney in her native Australia, she moved to electrophysiology, gaining her PhD between Australian National University and the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany. In Germany she was involved in developing the application of patch clamp techniques to brain slices working with Bert Sakmann and Arthur Konnerth. This enabled high resolution of synaptic currents between individual neurones in mammalian brain and subsequently led her to a postdoc at UCL and the discovery of the first fast purinergic synapse in the brain.

Webinar: Spatial Transcriptomics of Alzheimer's Disease Mouse Models with and Without Trem2R47H Mutation

Webinar Abstract: In this preliminary experiment, we used spatial transcriptomics to assess the gene expression profiles of microglia or astrocytes in relation to their distance from plaques. We compared 18-month-old APPKI mice (APPNL-F) which carry 2 mutations for familial Alzheimer’s disease to APPNL-F crossed with mice with the Trem2R47H mutation knocked in (APPTREM2KI; n=4 each genotype). In addition, we included Wild Type (WT) and Trem2R47H KI mice (n=2-3). The APPKI mice start to show amyloid plaques at about 9 months of age and by 18 months have a heavy plaque load. To do spatial transcriptomics with the GeoMx® Mouse Whole Transcriptome Atlas, microglia (Iba1 positive cells) areas of interest (AOIs) were divided into 3 groups: those touching plaques; those within an area of heavy plaque load (periplaque) and those far from plaques.

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