Скачать или смотреть Formation of a stack of serial sections obtained by FIB/SEM from the rat brain.

The video shows the formation of a stack of serial sections obtained by FIB/SEM from the neuropil of the rat somatosensory cortex (layer III). Synaptic junctions are then segmented and reconstructed. Asymmetric and symmetric synaptic junctions are shown in green and red respectively. The smallest spheres circumscribing the reconstructed synaptic junctions served to calculate the Feret’s diameters, which were used as an estimation of their size. An unbiased counting frame was also drawn to facilitate the quantification of the number of synapses per unit volume. The geometric centers or centroids of the synaptic junctions were also determined to indicate the spatial position of the synapses. The dimensions of the counting frame in this example were 7.16 x 4.58 x 3.98 µm. (Published in Cereb Cortex. 2014 Jun;24(6):1579-88).
Authors: Angel Merchán-Pérez, José-Rodrigo Rodríguez, Santiago González, Víctor Robles, Javier DeFelipe, Pedro Larrañaga and Concha Bielza
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