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Here are some steps for preparing a blood smear for microscopy:
1. Wear protective equipment
Wear gloves, a laboratory coat, and eye and face protection as indicated in your laboratory's SOP and safety manual.
2. Prepare the slide
Use a clean microscope slide with a frosted edge and label it with the sample identification.
3. Transfer the specimen
Place a small drop of blood in the center of the slide.
4. Spread the smear
Bring another slide at a 30–45° angle up to the drop of blood, allowing it to spread along the contact line. Then, quickly push the upper slide toward the unfrosted end of the lower slide to create a thin layer of cells. The smear should have a feathered edge and look roughly tongue-shaped.
5. Dry and fix
Allow the smear to air dry for a minute or so, then fix it by dipping it in absolute methanol.