Isolation Of Human Neutrophils From Whole Blood & Buffy Coats l Protocol Preview

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Isolation of Human Neutrophils from Whole Blood and Buffy Coats - a 2 minute Preview of the Experimental Protocol

Alan Y. Hsu, Zhicheng Peng, Hongbo Luo, Fabien Loison
Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, Department of Pathology; Mahidol University, Department of Microbiology, Faculty of Science;

This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

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