Скачать или смотреть Ampli-Fi: unlocking HiFi sequencing for low-input and challenging samples

What if your most critical samples—tiny insects, precious biopsies, or degraded FFPE tissues—have too little DNA for standard whole-genome sequencing? The PacBio Ampli-Fi protocol addresses this fundamental challenge, enabling high-quality HiFi sequencing from as little as 1 nanogram of input DNA.

Join experts from the Wellcome Sanger Institute, the USDA, and PacBio to explore how this streamlined and cost-effective workflow opens new frontiers in genomics. Learn how the Sanger team, led by Mike Quail, PhD, and Ksenia Krasheninnikova, applied the protocol in the Darwin Tree of Life project to generate high-quality assemblies from a wide range of species. Then, discover how Scott Geib, PhD, of the USDA-ARS uses Ampli-Fi to enable advanced applications like CiFi sequencing, achieving chromosome-scale assemblies from single insects to resolve complex structural variants.

This webinar provides a comprehensive look at a powerful tool for sequencing previously inaccessible samples and pushing the boundaries of genomic research.

00:00 Introducing the Ampli-Fi protocol
12:01 Early access testing for the Darwin Tree of Life project
23:01 Assembly and bioinformatics analysis of Ampli-Fi data
32:25 Enabling CiFi sequencing for chromosome-scale assembly
57:46 Q&A

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