To prepare an enzyme immobilisation and investigate its application

To prepare an enzyme immobilisation and investigate its application:
There are two steps to this experiment. First the enzyme (yeast cells) have to be immobilised. The second step was to investigate the immobilised enzyme in action.
Step one (enzyme immobilisation):
• Yeast is trapped using Sodium Alginate (gel) and Calcium Chloride (hardens gel).
• The Sodium alginate is mixed with water and yeast. This solution is then dropped slowly into a calcium chloride solution using a syringe to form beads.
• The beads are left to harden for 10 mins.
• The beads are then washed (to prevent contamination) to remove access Sodium alginate, Calcium Chloride and yeast.
Step two (Application of immobilised enzyme):
• Two tap funnels are set up. The immobilised yeast is added to one tap funnel and free yeast is added to another both containing a sucrose solution.
• The yeast (acts as enzyme) breaks down sucrose (substrate) to produce glucose (product).
• The tap funnel is opened to release some solution every minute and the solution is tested for the presence of glucose using Clinistix.
Result: Free yeast works faster but immobilised produces a pure product (clear) that can be easily removed, and the immobilised enzyme can be re-used.