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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples - a 2 minute Preview of the Experimental Protocol

Justin A. Courson, Paul T. Landry, Thao Do, Eric Spehlmann, Pascal J. Lafontant, Nimesh Patel, Rolando E. Rumbaut, Alan R. Burns
University of Houston, College of Optometry; DePauw University, Department of Biology; Michael E. DeBakey Veterans Affairs Medical Center, Center for Translational Research on Inflammatory Diseases (CTRID); Baylor College of Medicine, Children’s Nutrition Center;

This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.

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