Скачать или смотреть Characterization of LNPs via Density Matching AUC

Presented By: Amy Henrickson
Speaker Biography:
Amy Henrickson recently earned her Ph.D. in Biological Sciences with a focus on Biophysics in the lab of Dr. Borries Demeler, an expert in AUC. Amy's doctoral research focused on advancing and refining AUC methods for the characterization of AAVs and LNPs. Her outstanding academic achievements have been recognized through numerous scholarships and grants, including the prestigious NSERC CGS-D and CGS-M awards. Amy's groundbreaking work involved the application of multiwavelength AUC, D2O density matching, and analytical buoyant density equilibrium (ABDE) methods to assess cargo loading in LNPs and AAVs quantitatively. Her contributions also include the application of these innovative strategies to systems outside of gene therapy drugs, with an emphasis on the characterization of complex biopolymer interactions, including protein-nucleic acid and RNA-RNA interactions, in a large range of biomedical research projects. She has recently joined Beckman Coulter as a Market Development Manager in Analytical Development. In this capacity, she will continue working to improve the biophysical characterization of gene therapeutics, furthering her contributions to biophysics and analytical science.

Webinar: Characterization of LNPs via Density Matching AUC
Webinar Abstract:
Determining the purity of LNPs loaded with nucleic acids can be challenging due to their size heterogeneity. Here, we present analytical ultracentrifugation (AUC) methods that use multiwavelength and density matching with D2O. These methods allow us to measure the sedimentation and diffusion coefficient distributions of the LNPs, as well as the lipid and RNA components. Additionally, the density matching method allows us to determine their partial specific volume distributions; since the densities of the RNA and lipids are substantially different, the measured density is directly proportional to the loading of nanoparticles. Allowing for the characterization and differentiation of empty LNPS and different RNA loading states.

Learning Objectives

Understand the challenges associated with determining the purity of LNPs loaded with nucleic acids due to their size heterogeneity.
Learn about the analytical ultracentrifugation (AUC) methods that use multiwavelength and density matching with D2O for measuring the sedimentation and diffusion coefficient distributions of LNPs, as well as the lipid and RNA components.
Explore the application of density matching method in determining the partial specific volume distributions of LNPs, which allows for the characterization and differentiation of empty LNPs and different RNA loading states based on the measured density.

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