Interrupted mating gene mapping problems: Bacterial conjugation

Bacteria can transfer DNA by means of three natural processes. They are transformation, transduction, and conjugation. Among three, conjugation is unique cause it requires direct contact between the donor bacterium and the recipient. Donor cells generally transfer F plasmid to the recipient. F plasmid can also be integrated into the chromosome and when integrated it can able to transfer chromosomal genes. Donor cell transfer chromosomal genes depending on the order of the genes and distance between the genes. This process helps us to map the bacterial genome. When we interrupt the mating process at a certain interval and examine the recipient for the gene which have entered at that time tells us the distance of that gene from the oriT.
Conjugation is studied in great detail in E. coli. In some national level examination, min mapping problems appeared frequently. So, I have solved some of them to clear the concept. I hope you will find this video helpful for your exam preparation. best of luck.