How to Prepare a Single-Cell Suspension from Mouse Brain Tissue

This technical video outlines a method to prepare a single-cell suspension from harvested mouse brain tissue to be used in subsequent cell separation. This method has been optimized for downstream isolation of microglia using the EasySep™ Mouse CD11b Positive Selection Kit II.

For more information on sample processing, cell counting, or cell separation using EasySep™, visit https://www.stemcell.com

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0:00 Preparation
1:14 Transfer Brain Tissue
1:47 Discard supernatant
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