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Staphylococcus saprophyticus Colony Morphology on CLED Agar
Appearance:

Color: Colonies typically appear white to cream-colored.
Size: Medium-sized colonies, usually 1-2 mm in diameter.
Shape: Round with smooth, well-defined edges.
Elevation: Slightly raised or convex.
Lactose Fermentation:

Indicator: CLED agar contains bromothymol blue as a pH indicator.
Fermentation: S. saprophyticus does not ferment lactose, so colonies do not cause a color change in the medium.
Texture:

Surface: Smooth and glossy.
Consistency: Buttery or creamy.
Growth:

Speed: Moderate growth within 24-48 hours.
Pattern: Uniform distribution if evenly streaked.
Other Characteristics:

Odor: Mild, not distinctive.
Pigmentation: No pigmentation other than the white to cream color.
Selective and Differential Properties:

Selectivity: CLED agar supports the growth of both Gram-positive and Gram-negative bacteria.
Differentiation: Differentiates lactose fermenters (yellow colonies) from non-lactose fermenters (blue-green or unchanged medium).
Diagnostic Features:

Coagulase Test: Negative, distinguishing it from Staphylococcus aureus.
Novobiocin Sensitivity: Resistant, which helps differentiate it from other coagulase-negative staphylococci, particularly Staphylococcus epidermidis.
Clinical Relevance:

Urinary Tract Infections: S. saprophyticus is a common cause of UTIs, especially in young women.
Pathogenic Potential: Can cause UTIs and is commonly isolated from urine samples.
Understanding these characteristics aids in the accurate identification and differentiation of Staphylococcus saprophyticus from other bacteria in clinical samples, particularly urine specimens.

Keywords
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