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Today In this video I covered the Remaining important questions of Instrumental method of analysis B Pharm 7th with Pdf solution. (Part-3)
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Instrumental method of analysis Important questions Part-1 👇🔥
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• Part-2। Instrumental method of analys...
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Instrumental method of Analysis
Unit -1
UV Visible spectroscopy: Electronic transitions, chromophores, auxochromes, spectral shifts,solvent effect on absorption spectra, Beer and Lambert's law, Derivation and deviations.
Instrumentation -
Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube,
Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications- Spectrophotometric titrations, Single component and multi component analysis.
Fluorimetry: Theory, Concepts of singlet, doublet and triplet electronic states, internal and
external conversions, factors affecting fluorescence,
quenching, instrumentation and applications.
Unit-2
IR spectroscopy: Introduction, fundamental modes of vibrations in poly atomic molecules,
sample handling, factors affecting vibrations.
Instrumentation- Sources of radiation, wavelength selectors, detectors - Golay cell,
Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.
Flame Photometry- Principle, interferences, instrumentation and applications.
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications.
Nephelo turbidimetry- Principle, instrumentation and applications.
Unit-3
Introduction to chromatography: Adsorption and partition column chromatography- Methodology, advantages, disadvantages
and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications.
Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications.
Unit-4
Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature
programming, advantages, disadvantages and applications.
High performance liquid chromatography (HPLC)- Introduction, theory, instrumentation,
advantages and applications.
Unit-5
Ion exchange chromatography- Introduction, classification, ion exchange resins, properties,
mechanism of ion exchange process, factors affecting ion exchange, methodology and
applications. Gel chromatography- Introduction, theory, instrumentation and applications.
Affinity chromatography- Introduction, theory, instrumentation and applications.
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