Скачать или смотреть Cell-based Immunofluorescence to detect Autoantibody for NMDA Receptor | Protocol Preview

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A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood - a 2 minute Preview of the Experimental Protocol

Chia-Hsiang Chen, Yu-Syuan Chang
Chang Gung Memorial Hospital-Linkou, Department of Psychiatry; Chang Gung University, Department and Graduate Institute of Biomedical Sciences;

We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.

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