Qualitative Western Blotting | Meeting Western Blot Publication and Grant Funding Requirements

Introduction to Western Blots
Western blots are used to identify target proteins from a mixture of proteins in a biological sample. There are three types of Western blots:

- Qualitative
- Semi-quantitative
- Quantitative

Qualitative Western Blots
Qualitative Western blots are a visual assessment that test the presence or absence of a target protein. This form of blot can also be used to detect protein expression changes due to experimental manipulations, or to study DNA-protein interactions via Southwestern blotting.

References
Southwestern Blotting Assay Paper
https://www.ncbi.nlm.nih.gov/pmc/arti...

Western Blot Normalization Handbook
https://www.licor.com/documents/gtk1a...

About LICORbio
LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses.

For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system.

LICORbio Website
https://www.licorbio.com/

Reagent Store
https://www.licor.com/bio/reagents

Image Studio™ Software
https://www.licor.com/image-studio

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