Hybridoma Technology to Generate Monoclonal Antibodies | Protocol Preview

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Generation of Murine Monoclonal Antibodies by Hybridoma Technology - a 2 minute Preview of the Experimental Protocol

Pamela Holzlöhner, Katja Hanack
University of Potsdam, Department of Biochemistry and Biology;

An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.

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