Скачать или смотреть Efficient Method For Adenovirus Production l Protocol Preview

Watch the Full Video at https://www.jove.com/v/61691/an-effic....

An Efficient Method for Adenovirus Production - a 2 minute Preview of the Experimental Protocol

Madalina Dumitrescu, Violeta Georgeta Trusca, Ioana Madalina Fenyo, Anca Violeta Gafencu
Institute of Cellular Biology and Pathology "Nicolae Simionescu",;

Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments