Enzyme | Biochemistry | Pranav Kumar | CSIR NET | GATE | DBT | ICMR | IIT JAM | Pathfinder Academy

In this video, we'll delve into the topic of enzymes in biochemistry with Pranav Kumar from Pathfinder Academy. Get ready to ace exams like CSIR NET, GATE, DBT, ICMR, and IIT JAM with the insights shared in this comprehensive video.


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00:00 – Enzyme introduction
00:24 - What we will learn in this topic
01:23 - Enzyme
05:05 - Chemical reaction
07:43 - Spontaneous reaction
20:12 - Spontaneous reaction and rate of reaction
22:15 - Enzymes are highly specific
25:39 - Enzyme
30:19 - Catalytic RNA | Ribozyme
37:07 - Naming of enzymes
39:09 - Classification of enzymes
42:00 - Enzyme classes
47:43 - Synthase and Synthetase
49:04 - EC number
54:32 - Oxidoreductases
54:49 - Active site of the enzyme
56:23 - Interactions between substrate and enzyme
59:53 - Specificity of enzyme-substrate interactions
01:02:05 - Enzyme-substrate interactions
01:07:02 - Transition-state theory
01:09:47 - Transition-state
01:16:15 - Transition-state and free energy
01:20:07 - Activation energy
01:23:37 - What is activation energy
01:25:33 - Activation energy and reaction rate
01:31:32 - How enzymes operate
01:37:57 - How do enzyme lowers the activation energy?
01:40:06 - Maximal binding energy
01:42:42 - Enzyme kinetics
01:44:08 - Rate (velocity) of reaction and substrate concentrations
01:50:41 - Rate (velocity) of an enzyme-catalyzed reaction and substrate concentrations
01:52:51 - Rate of reaction and enzyme concentrations
01:54:11 - Order of reaction and substrate concentrations
01:56:13 - Michaelis and Menten equation
02:01:50 - Substrate and Product
02:14:08 - Michaelis and Menten equation
02:19:23 - Michaelis–Menten equation
02:35:33 - Michaelis constant
02:38:18 - Michaelis constant, Km
02:49:14 - Dissociation constant
02:53:57 - Vmax
02:59:02 - Turnover number (Catalytic constant)
03:02:12 - Specificity constant
03:09:20 - Lineweaver-Burk plot : why
03:12:58 - Lineweaver-Burk plot
03:21:24 - Hanes–Woolf plot
02:24:36 - Eadie-Hofstee plot