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Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells - a 2 minute Preview of the Experimental Protocol

Henrik D. Møller, Rasmus K. Bojsen, Chris Tachibana, Lance Parsons, David Botstein, Birgitte Regenberg
University of Copenhagen, Department of Biology; Technical University of Denmark, National Veterinary Institute; Group Health Research Institute,; Princeton University, Lewis-Sigler Institute for Integrative Genomics; Calico Life Sciences LLC,;

This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.

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