Скачать или смотреть SGD On Urine Analysis & Biochemistry Viva: Benedict’s, Rothera’s & Heat Coagulation Tests | MBBS

In this comprehensive practical session for the 2025-2026 MBBS batch at Santiniketan Medical College & Hospital, we delve into the qualitative and semi-quantitative analysis of abnormal urine constituents. This video is an excellent resource for medical students preparing for their practical exams and viva voce.

Key Topics & Highlights:

Benedict’s Test Deep Dive: Why it is called a semi-quantitative test and the clinical significance of a false-positive result [13:50]. We explore the concept of renal threshold for glucose (180 mg/dL) and why a negative Benedict’s test doesn’t always rule out diabetes [22:35].

Rothera’s Test for Ketone Bodies: A step-by-step demonstration of the test principle involving sodium nitroprusside in an alkaline medium. The instructor explains why it detects acetone and acetoacetate but not beta-hydroxybutyrate [33:43].

Heat Coagulation Test: Understanding the principle of protein denaturation by heat and the specific roles of the upper 1/3 and lower 2/3 of the test tube [16:34].

Diabetes Diagnostic Criteria: A review of Fasting Plasma Glucose (FPG) levels and the importance of HbA1c as a sophisticated confirmatory parameter [24:45] [28:08].

Metabolic Pathways: Discussion on the precursors of ketone bodies (Acetyl-CoA), oxidative decarboxylation of pyruvate, and the TCA cycle [01:24] [04:14].

Stereochemistry in Metabolism: The vital difference between D-glucose and L-glucose, and why the body prefers L-amino acids but D-carbohydrates [36:35].

Clinical & Lab Skills:

Titration & Reagent Handling: Observations on how strong acids (like acetic acid) affect Benedict’s reagent reactivity [08:52].

Viva Strategies: Tips on answering with confidence, using correct units (mg/dL), and interpreting "false-negative" vs. "false-positive" results.

Negative Control: Seeing what a truly negative Rothera’s test looks like to avoid misinterpretation in the lab [46:55].


SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: BIOCHEMISTRY by Dr. Karan Pal Singh
(MBBS Batch: 2025-26)
Conducted on: 07-01-26