Скачать или смотреть Transcripts Mapping by 3 prime Rapid Amplification of CDNA Ends | Protocol Preview

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Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer - a 2 minute Preview of the Experimental Protocol

Chioniso Patience Masamha, Zachery Todd
Butler University, Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences;

The two different 3' rapid amplification of cDNA ends (3' RACE) protocols described here make use of two different DNA polymerases to map sequences that include a segment of the open reading frame (ORF), the stop codon, and the entire 3' UTR of a transcript using RNA obtained from different cancer cell lines.

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