PCR | Polymerase Chain Reaction | Video 3

Описание к видео PCR | Polymerase Chain Reaction | Video 3

In 1986, a technique, called the polymerase chain reaction (PCR), was developed. PCR is a rapid method of DNA cloning that extends the power of recombinant DNA and in many cases eliminates the need to use host cells for cloning. PCR is a method of choice for many applications, whether in molecular biology, human genetics, evolution, development, conservation, or forensics. PCR Reagents are pre-requisite of reaction. Reagents include Template DNA, Primers, DNTP's, Taq Polymerase, PCR Buffer and MgCl2 which is a cofactor for Taq polymerase.
PCR is performed in three steps.
1) Denaturation
2) Annealing
3) Extension or Elongation.

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