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Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents - a 2 minute Preview of the Experimental Protocol

Carol E. Soteropulos, Heather K. Hunt
University of Missouri, Department of Biological Engineering;

Biosensors interface with complex, biological environments and perform targeted detection by combining highly sensitive sensors with highly specific probes attached to the sensor via surface modification. Here, we demonstrate the surface functionalization of silica optical sensors with biotin using silane coupling agents to bridge the sensor and the biological environment.

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