Скачать или смотреть "Dev. of a plasmonic immunosensor for the det. of Vascular Endothelial Growth Factor" - Maria Garcia

GOMa Seminar 2024-I

"Development of a plasmonic immunosensor for the detection of Vascular Endothelial Growth Factor"

M. F. García-Avellaneda (CICESE, Baja California)
E. R. Méndez-Méndez (Laboratorio de Inmunología Molecular y Biotoxinas CICESE, Baja California)
A. F. Licea-Navarro (Laboratorio de Inmunología Molecular y Biotoxinas CICESE, Baja California)

Abstract
We reports an investigation on the implementation of a biosensor based on the excitation of surface plasmon-polaritons (SPPs) and the use of antibodies as recognition elements. Due to these characteristics, it belongs to the class of sensors called SPR (Surface Plasmon Resonance) immunosensors, which are known for their sensitivity, and for the specificity and high affinity they can have with the target protein (in our case, the vascular endothelial growth factor protein, VEGF). SPR sensors use the dependence of the properties of the SPPs excited on the surface of a metal film with the possible presence of agents or molecules that, by affinity, adhere to the surface. For the recognitio of the VEGF shark antibody sections (vNARS, variable domain of immunoglobulin new antigen receptor) produced in the Department of Biomedical Innovation of CICESE were used (Camacho-Villegas et al., 2018). We present details of the optical arrangement, the detection, and data processing systems. With the system employed, the VEGF was detected in concentrations as low as 150 ng/mL, which represents ou detection limit. The study shows the viability of using vNARs as biorecognition agents in SPR systems.

Keywords: Biosensor, Surface plasmon resonance, Sharks antibodies, VEGF.