Principle of Chemiluminecence immunoaasy method.

Chemiluminescence (CL) is defined as the emission of electromagnetic radiation caused by a chemical reaction to produce light.

Chemiluminescence immunoassay (CLIA) is an assay that combine chemiluminescence technique with immunochemical reactions. Similar with other labeled immunoassays (RIA, FIA, ELISA), CLIA utilize chemical probes which could generate light emission through chemical reaction to label the antibody

Similar with other labeled immunoassays (RIA, FIA, ELISA), CLIA utilize chemical probes which could generate light emission through chemical reaction to label the antibody

There are two main steps Chemiluminecence Immunoassay reaction method
1. Immuno reaction technique.
2. Chemiluminescence technique.