Скачать или смотреть Dot Blot Analysis Tutorial: Complete Guide to Phoretix Array Software for Researchers

Struggling with dot blot analysis? Watch this comprehensive tutorial to learn how TotalLab's Phoretix Array software simplifies, speeds up, and standardizes dot blot quantification. Phoretix Array effectively eliminates common pain points like background noise, spot variability, and manual errors. Perfect for life scientists, researchers, and lab techs working with dot blots, ELISAs, and array experiments.
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IN THIS VIDEO, YOU'LL DISCOVER:
✓ How to import and prepare your dot blot images
✓ Setting up customizable grids and controls for precise quantification
✓ Background subtraction and normalization best practices
✓ Exporting accurate, reproducible data for your publications and reports
✓ Tips to avoid common pitfalls in dot blot analysis
✓ Why Phoretix Array is trusted by pharma and biotech labs worldwide

CHAPTERS
00:00 - Intro
00:42 - What is a dot blot?
01:45 - Image import and configuration
02:45 - Editing array images with the built-in image editor
02:57 - Image contrast adjustment
03:03 - How are measurements taken?
03:58 - 3D View of dot blot data
04:01 - Exporting 3D image for publication/report/presentation
04:13 - Exporting images for papers/reports/presentations
04:39 - Defining array grids for measurement + quantification
04:58 - Automatic array grid detection
05:28 - Editing existing grids
05:48 - Automatic 96 and 384 well plate templates
05:52 - Adding multiple measurement grids for quantification and analysis
06:57 - Editing measurement grids
07:04 - Editing individual measurement wells
07:34 - Importing and exporting grid templates
08:00 - A guide on well sizes for measurement
09:30 - Measurement of square wells
09:51 - Array background subtraction
10:02 - Surface minimum background subtraction
10:45 - Edge average background subtraction
11:03 - Defining negative control wells
12:04 - Defining multiple negative control wells
12:25 - Image rectangle background subtraction
13:49 - No background subtraction
14:20 - Selecting which data you want to see
14:56 - Exporting results to CSV/Microsoft Excel or clipboard
15:26 - Normalising wells/results
16:57 - Quantity calibration and quantitation of array data
17:30 - Building calibration curve
17:44 - Selecting curve fit type
18:07 - Data export to CSV, Excel or PDF report
18:24 - Presence/Absence filter for arrays
19:35 - How to automate your analysis using protocols
20:02 - Sharing protocols across sites and team members
20:49 - Adding annotations to array images
22:00 - Free trial download

KEY FEATURES:
✓ Automatic array grid detection with 96 and 384 well plate templates
✓ Multiple background subtraction methods (surface minimum, edge average, negative controls)
✓ Customizable and shareable grid templates across teams
✓ Built-in image editor with contrast adjustment
✓ 3D visualization of dot blot data
✓ Flexible normalization and calibration options
✓ CSV, Excel, and PDF export capabilities
✓ Protocol automation for consistent workflows
✓ Presence/absence filtering
✓ Multi-site protocol sharing

IDEAL FOR:

Life science researchers
Biotech and pharma labs
Academic research institutions
Protein expression studies
Immunoassay analysis
ELISA and array experiments
Antibody screening
Quality control labs
Multi-site research organizations

TRY PHORETIX ARRAY FREE:
Try on your own dot blots: https://totallab.com/software/phoreti...
Learn more: https://totallab.com/software/phoreti...
Download free trial: https://www.totallab.com/phoretix-array
Contact us: https://www.totallab.com/contact

Whether you're new to dot blot analysis or want a scalable, validated workflow to boost your data quality, this step-by-step guide will save you time and improve confidence in your results.

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