The Basics of the Recombinant Lentivirus System

How do recombinant lentivirus systems work? Lentiviruses are members of the Retroviridae family of viruses, with HIV-1 being the most widely studied. Lentiviral vectors are able to deliver genes into a broad range of mammalian cell lines and tissue types and to infect both dividing and non-dividing cells. Recombinant lentivirus systems and lentiviral transfection/transduction are powerful tools and methods for gene delivery and gene therapy. Because they can stably integrate transgenes into the host genome, lentiviruses are unique tools for generating stable cell lines for long term expression. In this video we will introduce you to the basic structure and genome map of the HIV Lentivirus and explain the differences and advantages of using first, second, and third generation recombinant lentivirus packaging systems. In the recombinant system, the HIV lentiviral genome is split into multiple plasmids (e.g. lentiviral transfer plasmids, packaging plasmids and envelop plasmids) that are delivered separately to ensure the introduced lentivirus cannot replicate in host cells. Thus, though natural lentiviruses such as HIV, SIV, FIV, EIAV, and Visna can cause chronic and even fatal diseases, scientists have managed to reduce the biosafety risks to a minimal level and convert this group of viruses into a tool for research and treatment of diseases such as cancers.

For more information on the recombinant lentivirus expression system and for a list of the sources used, please visit:
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