HPLC Calibration Step by Step Procedure

Welcome to our comprehensive guide on High-Performance Liquid Chromatography (HPLC) calibration! 📊🔬 In this video, we walk you through the essential steps to calibrate your HPLC system effectively, ensuring the most accurate and reliable results for your analyses. Whether you're a beginner or an experienced professional, these tips and techniques will help you master the calibration process with confidence.

Timecodes:
00:00 intro
00:05 Why is Calibration Necessary?
00:27 Calibration parameters
00:51 D2 lamp energy check (detector)
01:36 Leakage test (by pressure drop)
02:10 Flow rate accuracy
03:54 Wavelength accuracy test for UV detector
05:08 Linearity of detector response
06:46 Calibration of injector & loop
7:48 Auto sampler injector calibration
09:13 Calibration for gradient program test
10:47 Column oven temperature
11:16 Carry over test


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