Microbial Colony Biofilms Preparation for Microscopic Analyis | Protocol Preview

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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis - a 2 minute Preview of the Experimental Protocol

William C. Cornell, Chase J. Morgan, Leslie Koyama, Hassan Sakhtah, Jennifer H. Mansfield, Lars E.P. Dietrich
Columbia University, Department of Biological Sciences; Columbia University, Department of Biology, Barnard College;

We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.

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