Deciphering Brain Mosaicism with Long-read Sequencing

Presented By: Tracy Bedrosian, Ph.D.

Speaker Biography: Tracy Bedrosian leads a laboratory in the Institute for Genomic Medicine at Nationwide Children's Hospital. Her group uses single-cell sequencing approaches to understand how brain somatic mutations contribute to neurodevelopmental disorders, including developmental brain malformations, epilepsy, and autism. She also serves as an Assistant Professor of Pediatrics at The Ohio State University.

Webinar: Deciphering Brain Mosaicism with Long-read Sequencing

Webinar Abstract: Somatic mutations that arise during development are increasingly recognized as contributors to neurodevelopmental disease. One challenge has been to determine how mosaic mutations contribute to cell lineages and affect the function of individual cells, which could have implications for disease manifestation. We integrated high-throughput single-cell 3’-RNA-sequencing with targeted Iso-Seq long-read RNA sequencing on the PacBio Sequel II platform to overlay single-cell genotype with cell-type and gene expression analyses. We applied this strategy to study the contribution of somatic mutations to developmental brain malformations in surgically-resected patient brain tissue.

Earn PACE Credits:
1. Make sure you’re a registered member of Labroots (https://www.labroots.com/ms/webinar/d...)
2. Watch the webinar on YouTube or on the Labroots Website (https://www.labroots.com/ms/webinar/d...)
3. Click Here to get your PACE credits: (https://www.labroots.com/credit/pace-...)

Labroots on Social:
Facebook:   / labrootsinc  
Twitter:   / labroots  
LinkedIn:   / labroots  
Instagram:   / labrootsinc  
Pinterest:   / labroots  
SnapChat: labroots_inc