Скачать или смотреть Preventing ex vivo activational confounds in single cell neuroscience studies [WEBINAR]

For more information on our neural cell workflow, visit our website. https://www.miltenyibiotec.com/lp/Cel... A long-standing concern for neuroscientists studying specific cell types in vivo is whether cell isolation from CNS tissue induces ex vivo activational artifacts that confound experimental interpretations. Here we present best practices for isolating glial and neuronal cells for downstream ‘omic analyses. We show that the inclusion of transcription and translation inhibitors during cell preparation minimizes ex vivo activation induced during enzymatic and mechanical dissociation. We also compare cell preparation and isolation techniques for various CNS cell types.

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