Скачать или смотреть 3D Imaging of a Rabbit Retina: High-Resolution Tissue Mapping

Rabbit retina in 3D. HOTLS imaging captures retina, choroid, and sclera intact , preserving native architecture and enabling cellular-resolution analysis beyond 2D histology.

This dataset presents a high-resolution 3D visualization of a cleared rabbit retina, captured intact with the Aurora™ 3Di Hybrid Open Top Light Sheet (HOTLS) microscope. By avoiding physical sectioning, the imaging preserves the true spatial architecture of the tissue.

Stains used:
🟣 TO-PRO-3 (Magenta, pseudocolor): nuclei
🟢 Eosin (Green, pseudocolor): cytoplasmic structures

The fly-through reveals the full thickness of the sample (~1 × 1 × 4 mm), including the retina, choroid, and sclera, all in their native spatial organization. This volumetric dataset supports accurate morphological and contextual analysis at cellular resolution, overcoming the constraints of 2D histology.

When combined with 3Dm data management and 3Dai AI-powered segmentation, the dataset enables quantification of:

Nuclear density

Layer-specific organization

Cellular distribution

Applications:

Ophthalmology research

Retinal disease modeling

Translational studies in vision science

This dataset demonstrates how 3D histology, digital pathology, and spatial biology provide deeper insight into ocular tissue organization and disease.

https://www.alpenglowbiosciences.com/...