Plant Isotope Labeling by 13C and 15N Labelling Chamber | Protocol Preview

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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling - a 2 minute Preview of the Experimental Protocol

Jennifer L Soong, Dan Reuss, Colin Pinney, Ty Boyack, Michelle L Haddix, Catherine E Stewart, M. Francesca Cotrufo
Colorado State University, Natural Resource Ecology Laboratory; USDA-ARS, Soil Plant Nutrient REsearch; Colorado State University, Department of Soil and Crop Sciences;

This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

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