Proteintech Protocols: Western Blotting

In this detailed tutorial, we'll walk you through the complete Western Blot protocol, a powerful technique used for protein detection and analysis. Whether you're a beginner or just need a refresher, this video covers everything you need to know to successfully perform a Western Blot in your lab.

In this video, we cover:

1. Sample Preparation
2. SDS PAGE
3. Transfer
4. Blocking
5. Antibody Incubations
6. Washing
7. Detection

Whether you're working with cell lysates, tissue samples, or recombinant proteins, this step-by-step guide will help you achieve clear, reproducible results. Subscribe for more Proteintech Protocols and research tips!

Tags: #WesternBlot #ProteinDetection #LabTutorial #SDSPAGE #molecularbiology #immunoblotting #researchskills

00:00 - Western Blotting
00:23 - Sample Preparation
01:09 - SDS PAGE
02:48 - Transfer
04:45 - Blocking
05:23 - Primary Antibody Incubation
06:14 - Washing
06:57 - Secondary Antibody Incubation
07:47 - Washing
08:48 - Detection