Скачать или смотреть Bibel lab/class GC-MS update - standards for our alphabet soup of a sample

GC-MS is a bit like trying to figure out what letter noodles and how much of each are in a bowl of alphabet soup. The gas chromatography (GC) part separates the noodles and then the mass spectrometry (MS) part gives you info that helps you identify them. When you’re dealing with a messy soup of a sample, it’s good to have standards to compare to – pure solutions you prepare of one type of noodle (molecule). Then you know when to expect it to come out of the GC step (retention time). Which helps you better fish out the noodle signal you’re looking for in the spectral data the MS gives you, where the data for different noodles/molecules may look very similar (if they have similar m/z and fragment ions).

So, I made some derivatized standards and am running them now with the method I worked out for the GC to get best separation of our molecular alphabet soup!

We’re getting the most success with a variation (we had to up the volume and lower the split) on this article, which was also our go-to for derivatization strategies:

Engel, B., Suralik, P., & Marchetti‐Deschmann, M. (2020). Critical considerations for trimethylsilyl derivatives of 24 primary metabolites measured by gas chromatography–tandem mass spectrometry. Separation Science Plus, 3(9), 407-418. https://doi.org/10.1002/sscp.202000025

We have some prominent peaks that show intriguing differences between samples that I can’t currently identify, so I’m trying MassBank search which is really cool, but not helping in this case. https://massbank.eu/MassBank/Search

Very open to any suggestions for peak identification!

More on optimizing and cute little vials:    • Bibel lab/class update: GC-MS (Optimizing ...  

More on the derivatization step: blog: https://bit.ly/gcmstest YouTube:    • Bibel lab update: Derivatization of metabo...  

Resources I’ve found helpful:

Moldoveanu, S. C. and David, V. (2019). Derivatization methods in GC and GC/MS. Gas Chromatography - Derivatization, Sample Preparation, Application. https://doi.org/10.5772/intechopen.81954

Engel, B., Suralik, P., & Marchetti‐Deschmann, M. (2020). Critical considerations for trimethylsilyl derivatives of 24 primary metabolites measured by gas chromatography–tandem mass spectrometry. Separation Science Plus, 3(9), 407-418. https://doi.org/10.1002/sscp.202000025

LibreTexts Chemistry 2.5E: GC Parameters, Lisa Nichols: https://chem.libretexts.org/Bookshelv...

LibreTexts Lab 5: Gas Chromatography/Mass Spectrometry (GC/MS) by Fawcettt et al. https://chem.libretexts.org/Courses/U...)

A Split Decision, John V. Hinshaw, LCGC Europe-12-01-2007, https://www.chromatographyonline.com/...

More about the Bibel lab:
official site: https://sites.google.com/stmarys-ca.e...
blog: https://thebumblingbiochemist.com/upd...

more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com