Скачать или смотреть Implementing shallow screening strategies to accelerate microbial strain improvement

Presented By:
Eileen Spindler

Speaker Biography:
Eileen Spindler is a Senior Director of Applications at Inscripta. Dr. Spindler is a cell biologist and an expert in the genome engineering space, having led multiple teams at Inscripta involved in building the microbial platform. She currently leads the Microbial Applications team in Boulder, Colorado, where they focus on demonstrating the potential of the Onyx® platform in functional genomics, genome biology, human health, and bioindustry. Dr. Spindler is actively involved in building partnerships and collaborations in the areas of automation and microbial genome engineering. She is a native Montanan who holds a B.S in Biochemistry and a Ph.D. in Molecular, Cellular and Developmental Biology from the University of Colorado.

Webinar:
Implementing shallow screening strategies to accelerate microbial strain improvement

Webinar Abstract:
The ability to move rapidly through the Design-Generate-Test-Learn (DGTL) cycle is essential for effective forward engineering of microbial phenotypes. The Onyx® platform is an automated CRISPR-based genome editing technology that enables rapid diversity generation. To optimize the DGTL cycle, effective diversity generation must be paired with an appropriate screening strategy for isolated genetic variants. Here we discuss the benefits of creating a higher edit diversity in cell populations while using a shallow screening approach with high-throughput and automated phenotyping systems such as Rapid Fire Mass Spec and plate-based fluorometric assays. We demonstrate the effectiveness of this approach using both metabolic and strain engineering examples, including a 14,000-fold improvement in lysine biosynthesis in E. coli and 4-fold increase in heterologous protein production in S. cerevisiae.

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