BMPR-IB+ Adipose-Derived Stromal Cells Isolation | Protocol Preview

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Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model - a 2 minute Preview of the Experimental Protocol

Clement D. Marshall, Elizabeth R. Zielins, Elizabeth A. Brett, Charles P. Blackshear, Michael S. Hu, Tripp Leavitt, Leandra A. Barnes, H. Peter Lorenz, Michael T. Longaker, Derrick C. Wan
Stanford University School of Medicine, Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery;

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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