Co-Culture Models: Pseudomonas aeruginosa Biofilms: Live Human Airway Cells Grown l Protocol Preview

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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells - a 2 minute Preview of the Experimental Protocol

Sophie Moreau-Marquis, Carly V. Redelman, Bruce A. Stanton, Gregory G. Anderson
Dartmouth College, Department of Physiology; Indiana University Purdue University Indianapolis, Department of Biology;

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

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