RNA seq Analysis Workflow | From fastq to read counts using featurecounts

RNA Seq FeatureCounts Tutorial

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Materials
_________________________________________________________________________________

Original tutorial: https://training.galaxyproject.org/tr...

Install Tools
Anaconda
Linux    • Install , Configure and Run Anaconda ...  
MacOS
   • Install, Configure and Run Anaconda i...   - GUI Installer
   • Install, Configure and Run Anaconda i...   - Command line installer

STAR
anaconda:    • STAR RNA Seq Download and Install | A...  
binaries:   • STAR RNA Seq Download and Install | B...  
compile from source:    • STAR RNA Seq Download and Install | C...  

fastqc
binaries:    • Fastqc Linux Install and Usage (Comma...  
conda:    • fastqc linux install and usage (conda...  


featureCounts
binaries:    • RNA Seq Tools | How to Install and Ru...  
conda:    • RNA Seq |  FeatureCounts Linux Instal...  

RNA seq analysis playlist   • Плейлист  

STAR Documentation
github: https://github.com/alexdobin/STAR
manual: https://github.com/alexdobin/STAR/blo...


Chapters

00:03 Intro
00:11 Outline of this tutorial
01:32 PC Requirement
03:49 Download Example Data
18:56 Install tools using anaconda
21:15 Activate the environment
22:21 Generate Index
27:32 Mapping
42:50 FeatureCount
49:22 Aggreate feature count report



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