Chromatography Overview

Chromatography Overview

Chromatography is a separation technique based on the differential migration of solutes in a mixture through a mobile phase and a stationary phase. The types of chromatography include liquid chromatography (LC), gas chromatography (GC), thin-layer chromatography (TLC), and paper chromatography, each differing in the phases used. For instance, the mobile phase can be a liquid or a gas, while the stationary phase may be solid or liquid. Chromatography is utilized across various fields for its ability to separate and identify chemical compounds. It is particularly valuable in biochemical research for analyzing biological compounds, in the petroleum industry for hydrocarbon analysis, and in environmental studies for detecting pesticides. Its applications extend to forensic science for substance identification and in healthcare for detecting therapeutic and abused drugs. This technique is favored for its versatility, allowing the separation of molecules ranging from small to very large, and for its sensitivity, capable of detecting substances at extremely low levels.

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