Скачать или смотреть Aseptic Transfer of Liquids | Microbiology Lab

Microorganisms are everywhere - in the air, soil, and human body as well as on inanimate surfaces like laboratory benches and computer keyboards. The ubiquity of microbes creates a copious supply of potential contaminants in a laboratory. To ensure experimental success, the number of contaminants on equipment and work surfaces must be minimized. Common among many experiments in microbiology are techniques involving the measurement and transfer of cultures containing bacterial cells or viral particles.

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Aseptic technique refers to a set of routine procedures done to prevent sterile solutions and cultures from becoming contaminated by unwanted microorganisms in the laboratory. Such techniques are essential for experiments that require growing cells. Although a work setting that is completely sterile cannot be achieved, procedures such as disinfecting laboratory surfaces, creating a sterile field using a Bunsen burner, limiting exposure of uncapped cultures and media to the air, sterilizing materials such as bottles, tubes and glass pipettes, and avoiding contact of sterile instruments with non-sterile surfaces reduces the possibility of contaminating solutions and cultures in an experiment. The goal is for these precautionary procedures to become second nature; this comes with training and practice while working in a laboratory.

Volume transfers with sterile solutions and cultures using instruments such as serological pipettes and micropipettors are one of many types of routine techniques done in a laboratory. Different experimental applications call for instruments capable of transferring distinct, yet precise and accurate, volumes. Serological pipettes are used in microbiology laboratories to prepare cell cultures requiring media preparations involving milliliter volumes, while micropipettors are essential to molecular biology experiments that need only microliter amounts of solutions. When aseptic technique is practiced with these instruments, contamination is minimized during volume transfers regardless of amount of liquid or type of experiment.

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Source:
http://www.ncbi.nlm.nih.gov/pmc/artic...