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Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform - a 2 minute Preview of the Experimental Protocol

Ignacio Risueño, Leticia Valencia, Miguel Holgado, Jose L. Jorcano, Diego Velasco
Universidad Carlos III de Madrid (UC3M), Department of Bioengineering and Aerospace Engineering; Universidad Politécnica de Madrid, Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology; CIEMAT, Division of Epithelial Biomedicine; Instituto de Investigación Sanitaria Gregorio Marañón,;

Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.

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