Tips and Techniques for Culturing Organoids

The currently available preclinical cancer models are often inadequate for studying cancer biology, developing personalized therapeutics, and identifying unique biomarkers. To address this deficit, ATCC has collaborated with the Human Cancer Model Initiative (HCMI) to launch almost 150 patient-derived models, featuring over 80 organoids. The clinical and sequencing data for these advanced models are available through a linked portal to the HCMI searchable catalog and the Genomic Data Commons (GDC) data portal. In addition to providing the models, ATCC supports scientists who are new to culturing these complex microtissues by offering organoid culture protocols and complete medium formulations. In this webinar we present step-by-step instructions that thoroughly demonstrate the process of thawing, expanding, and cryopreserving organoids.