Скачать или смотреть Visualizing the cell cycle clock using novel single fluorophore biosensors - Julia Kamenz - NGBS2024

Visualizing the cell cycle clock using novel single fluorophore biosensors
Speaker: Julia Kamenz, University of Groningen, Netherlands
Chair: Geoff Holdgate, AstraZeneca, UK
Co-Chair: Chris Johnson, MRC Laboratory of Molecular Biology, UK

Julia Kamenz describes how oscillators are ubiquitous in biology. She focuses her investigations on the cell cycle showing how the synchronous cell division in the early Xenopus embryo can be recapitulated in cell extracts in oil droplets. To understand the activators and negative feedback that give rise to these oscillations, she has development two novel biosensors. Both are based on circularly permuted proteins containing a phosphate binding domain that will bind to a specific kinase target protein sequence upon its phosphorylation giving rise to a change in either intrinsic protein fluorescence in the case of GFP or an added fluorescent dye in HaloTag. Using the later system, she was able to detail the changes in kinase activity over a large number of droplets over many cycles. She emphasizes that the multiple phases observed within each cycle are not only driven by kinase activation but by phosphatase activity as well.

Read more about Julia Kamenz’s work here: https://www.kamenzlab.com/

You can find more about the Next Generation Biophysics Symposium 2024 here: https://www3.mrc-lmb.cam.ac.uk/sites/...

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