Beer Lambert's Law, Absorbance & Transmittance - Spectrophotometry, Basic Introduction - Chemistry

This chemistry video tutorial provides a basic introduction into spectrophotometry and beer lambert's law also known as beer's law. It briefly explains how a spectrophotometer works. It uses a light source and a monochromator which selects a specific wavelength of light using a prism or a grating. The amount of light striking the sample is the incident light Io and the intensity of light picked up by the detector is I. The ratio of I to Io is known as the transmittance. The absorbance is the negative log of the transmittance. As the transmittance increases, the absorbance decreases. Beer's Lambert law is associated with the equation A = elc where A is the absorbance, e is the molar absorptivity, l is the pathlength in cm and c is the concentration in Molarity or moles per liter. This video contains plenty of examples and practice problems calculating the absorbance, molar absorptivity and unknown concentration using beer lambert's law. This video explains how to determine the linear equation in slope intercept form given a data table of absorbance and concentration. It also explains how to determine the unknown concentration using proportions.

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