Скачать или смотреть How to Purify DNA from an Agarose Gel (E-Gel CloneWell II)

Learn how to purify DNA from an agarose gel using the Invitrogen E-Gel™ CloneWell™ II cassette on the E-Gel™ Power Snap system: https://www.thermofisher.com/us/en/ho...

No spin columns, no extra cleanup kits. Load your sample, run the gel, then pipette the band directly into a tube for fast, clean recovery.

What you’ll learn:
1. When to use CloneWell II for band-to-tube DNA purification
2. How to set up the E-Gel Power Snap and CloneWell II cassette
3. How to recover target DNA bands quickly with minimal handling
4. Tips to improve yield and reduce carryover

Materials used:
1. E-Gel™ CloneWell™ II agarose gel cassette
2. E-Gel™ Power Snap system
3. DNA sample, loading buffer
4. Pipette + tips, microcentrifuge tubes

Steps overview:
1. Prepare cassette and load samples
2. Run gel and visualize target band
3. Pipette DNA directly from the CloneWell well into a tube
4. Downstream use (cloning, PCR, sequencing)

See additional resource for the smartest way to gel-purify your DNA: https://documents.thermofisher.com/TF...

FAQs
1. Does this replace column-based gel extraction?
For many workflows, yes—CloneWell II enables direct band recovery without a separate cleanup kit.
2. What sizes are best recovered?
Typical gel-purification fragment ranges apply; aim for bright, well-resolved bands.

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