Fluorescent Dye and Multiplexing Options | Fluorescence: Secondary Antibody Incubation

Fluorescent Dye and Multiplexing Options
The main advantage of using dyes for detection, as opposed to enzymes in chemiluminescent detection, is that the intensity of the signals will not be affected by enzymatic activity, and will therefore be stable. This is in stark contrast to the dynamic signals produced by an enzymatic reaction.

If the imaging system has multiple detectors tuned to different specific wavelengths, it is possible to multiplex the detection, that is to detect multiple different targets at the same time even if the bands co-migrate and overlap.

Lambda U Course: Linear Range for Quantitative Western Blots


About LICORbio
LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses.

For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system.

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https://www.licor.com/image-studio

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