Protein Analysis: The Bradford Assay & Spectrophotometry

Proteins are expressed from our genetic blueprint to perform specific functions in the cell, and even a small difference in protein concentration can lead to defect and disease. When we perform protein experiments that try to replicate the conditions inside living cells within a test tube, we need to know exactly how much protein is present under different conditions. Today we will build on our previous discussions on protein analysis to talk more broadly about how we can measure protein concentration in any molecular biology experiment using spectrophotometry and the Bradford Assay. All of the techniques we will talk about in this video are used to measure protein concentration before running your SDS-PAGE experiment.

My name is Jack Wang, a microbiologist and science educator, and the 2020 Australian University Teacher of the Year. You can find out more about my work here: https://jackwang.com.au
Blog entry accompanying this video: https://jackwang.com.au/blog/applesan...

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Our previous videos on protein analysis:
SDS-Page -    • Protein Analysis: SDS-PAGE  
Western Blotting -    • Protein analysis: Western Blotting  

#biolabcollective #protein #laboratory #science #microbiology

00:00 Introduction
01:21 Absorbance at 280nm
02:07 Nanodrop
04:14 The Bradford Assay
04:56 Conclusion