Скачать или смотреть How to Prepare Chemically Competent E. coli Cells | Wet Lab Protocol

Welcome to The Wet Lab Journal! In this detailed tutorial, we walk you through the complete preparation of chemically competent E. coli cells using the calcium chloride (CaCl₂) method — one of the most widely used techniques in molecular biology for enabling bacterial transformation.

Competent cells are E. coli cells treated to become more permeable to DNA, allowing them to take up plasmids during transformation experiments. This process is a critical step in genetic engineering, cloning, and recombinant protein expression workflows.

In this video, you’ll learn:

How to grow and prepare an overnight E. coli culture

The importance of keeping everything cold (timing and temperature matter!)

How calcium chloride disrupts the cell wall to make the cells DNA-permeable

Tips on harvesting cells without losing viability

How to aliquot and store your cells for long-term use

Whether you’re a student setting up your first transformation or a researcher refining your workflow, this protocol will give you clear, visual guidance and helpful tips to avoid common pitfalls.

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